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tlr4 polyclonal antibody  (Proteintech)


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    Proteintech tlr4 polyclonal antibody
    Effect of FP and SFP on the <t>TLR4/MyD88</t> signaling in blood vessels. (A) Representative images from immunohistochemical staining of TLR4 in blood vessels (×200). (B, C, E) Western blotting to determine the levels of TLR4, MyD88, NFκB p65 and its phosphorylation, IκBα and its phosphorylation, and IKKβ and its phosphorylation in blood vessels. (D) Changes in serum TNF-α and IL-6 levels. (F) Relative expression of TLR4, MyD88, NFκB p65, IκBα, and IKKβ mRNA. Compared with the WKY group, # p < 0.05, ## p < 0.01; compared with the SHR group, * p < 0.05, ** p < 0.01; compared with the FP group, ▲ p < 0.05, ▲▲ p < 0.01, n = 3.
    Tlr4 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tlr4+polyclonal+antibody/TLR4+Antibody/pmc13020082-49-0-26
    Average 96 stars, based on 1323 article reviews
    tlr4 polyclonal antibody - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy"

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy

    Journal: Ultrasonics Sonochemistry

    doi: 10.1016/j.ultsonch.2026.107829

    Effect of FP and SFP on the TLR4/MyD88 signaling in blood vessels. (A) Representative images from immunohistochemical staining of TLR4 in blood vessels (×200). (B, C, E) Western blotting to determine the levels of TLR4, MyD88, NFκB p65 and its phosphorylation, IκBα and its phosphorylation, and IKKβ and its phosphorylation in blood vessels. (D) Changes in serum TNF-α and IL-6 levels. (F) Relative expression of TLR4, MyD88, NFκB p65, IκBα, and IKKβ mRNA. Compared with the WKY group, # p < 0.05, ## p < 0.01; compared with the SHR group, * p < 0.05, ** p < 0.01; compared with the FP group, ▲ p < 0.05, ▲▲ p < 0.01, n = 3.
    Figure Legend Snippet: Effect of FP and SFP on the TLR4/MyD88 signaling in blood vessels. (A) Representative images from immunohistochemical staining of TLR4 in blood vessels (×200). (B, C, E) Western blotting to determine the levels of TLR4, MyD88, NFκB p65 and its phosphorylation, IκBα and its phosphorylation, and IKKβ and its phosphorylation in blood vessels. (D) Changes in serum TNF-α and IL-6 levels. (F) Relative expression of TLR4, MyD88, NFκB p65, IκBα, and IKKβ mRNA. Compared with the WKY group, # p < 0.05, ## p < 0.01; compared with the SHR group, * p < 0.05, ** p < 0.01; compared with the FP group, ▲ p < 0.05, ▲▲ p < 0.01, n = 3.

    Techniques Used: Immunohistochemical staining, Staining, Western Blot, Phospho-proteomics, Expressing

    Related Articles

    Immunohistochemical staining:

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy.
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Protection Mechanisms Underlying Oral Administration of Chlorogenic Acid against Cadmium-Induced Hepatorenal Injury Related to Regulating Intestinal Flora Balance.
    Article Snippet: Cadmium (Cd) is a heavy metal, which is widely used in the industry and daily life.. It has a long half-life, so large amounts of Cd can accumulate in humans and become toxic.. Chlorogenic acid (CGA) can eliminate free radicals and inhibit lipid peroxidation and is mainly used to prevent metal toxicity.

    Article Title: Structure characterization and in vitro immunomodulatory effects of a novel galactoglucomannan in cultivated Chinese cordyceps (Cordyceps sinensis).
    Article Snippet: Chinese cordyceps (Cordyceps sinensis) is a rare medicinal fungus with significant therapeutic value, and its cultivated variants have garnered increasing attention in recent years.. However, detailed structural and functional insights into cultivated Chinese cordyceps (CCC) polysaccharides remain limited.. In this study, a novel galactoglucomannan (CSWP-80) with a molecular weight (Mw) of 26.2 kDa was extracted and purified from CCC.

    Staining:

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy.
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Protection Mechanisms Underlying Oral Administration of Chlorogenic Acid against Cadmium-Induced Hepatorenal Injury Related to Regulating Intestinal Flora Balance.
    Article Snippet: Cadmium (Cd) is a heavy metal, which is widely used in the industry and daily life.. It has a long half-life, so large amounts of Cd can accumulate in humans and become toxic.. Chlorogenic acid (CGA) can eliminate free radicals and inhibit lipid peroxidation and is mainly used to prevent metal toxicity.

    Article Title: Structure characterization and in vitro immunomodulatory effects of a novel galactoglucomannan in cultivated Chinese cordyceps (Cordyceps sinensis).
    Article Snippet: Chinese cordyceps (Cordyceps sinensis) is a rare medicinal fungus with significant therapeutic value, and its cultivated variants have garnered increasing attention in recent years.. However, detailed structural and functional insights into cultivated Chinese cordyceps (CCC) polysaccharides remain limited.. In this study, a novel galactoglucomannan (CSWP-80) with a molecular weight (Mw) of 26.2 kDa was extracted and purified from CCC.

    Western Blot:

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy.
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Protection Mechanisms Underlying Oral Administration of Chlorogenic Acid against Cadmium-Induced Hepatorenal Injury Related to Regulating Intestinal Flora Balance.
    Article Snippet: Cadmium (Cd) is a heavy metal, which is widely used in the industry and daily life.. It has a long half-life, so large amounts of Cd can accumulate in humans and become toxic.. Chlorogenic acid (CGA) can eliminate free radicals and inhibit lipid peroxidation and is mainly used to prevent metal toxicity.

    Article Title: Structure characterization and in vitro immunomodulatory effects of a novel galactoglucomannan in cultivated Chinese cordyceps (Cordyceps sinensis).
    Article Snippet: Chinese cordyceps (Cordyceps sinensis) is a rare medicinal fungus with significant therapeutic value, and its cultivated variants have garnered increasing attention in recent years.. However, detailed structural and functional insights into cultivated Chinese cordyceps (CCC) polysaccharides remain limited.. In this study, a novel galactoglucomannan (CSWP-80) with a molecular weight (Mw) of 26.2 kDa was extracted and purified from CCC.

    Phospho-proteomics:

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy.
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Protection Mechanisms Underlying Oral Administration of Chlorogenic Acid against Cadmium-Induced Hepatorenal Injury Related to Regulating Intestinal Flora Balance.
    Article Snippet: Cadmium (Cd) is a heavy metal, which is widely used in the industry and daily life.. It has a long half-life, so large amounts of Cd can accumulate in humans and become toxic.. Chlorogenic acid (CGA) can eliminate free radicals and inhibit lipid peroxidation and is mainly used to prevent metal toxicity.

    Article Title: Structure characterization and in vitro immunomodulatory effects of a novel galactoglucomannan in cultivated Chinese cordyceps (Cordyceps sinensis).
    Article Snippet: Chinese cordyceps (Cordyceps sinensis) is a rare medicinal fungus with significant therapeutic value, and its cultivated variants have garnered increasing attention in recent years.. However, detailed structural and functional insights into cultivated Chinese cordyceps (CCC) polysaccharides remain limited.. In this study, a novel galactoglucomannan (CSWP-80) with a molecular weight (Mw) of 26.2 kDa was extracted and purified from CCC.

    Expressing:

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy.
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Protection Mechanisms Underlying Oral Administration of Chlorogenic Acid against Cadmium-Induced Hepatorenal Injury Related to Regulating Intestinal Flora Balance.
    Article Snippet: Cadmium (Cd) is a heavy metal, which is widely used in the industry and daily life.. It has a long half-life, so large amounts of Cd can accumulate in humans and become toxic.. Chlorogenic acid (CGA) can eliminate free radicals and inhibit lipid peroxidation and is mainly used to prevent metal toxicity.

    Article Title: Structure characterization and in vitro immunomodulatory effects of a novel galactoglucomannan in cultivated Chinese cordyceps (Cordyceps sinensis).
    Article Snippet: Chinese cordyceps (Cordyceps sinensis) is a rare medicinal fungus with significant therapeutic value, and its cultivated variants have garnered increasing attention in recent years.. However, detailed structural and functional insights into cultivated Chinese cordyceps (CCC) polysaccharides remain limited.. In this study, a novel galactoglucomannan (CSWP-80) with a molecular weight (Mw) of 26.2 kDa was extracted and purified from CCC.

    Positive Control:

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy.
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Protection Mechanisms Underlying Oral Administration of Chlorogenic Acid against Cadmium-Induced Hepatorenal Injury Related to Regulating Intestinal Flora Balance.
    Article Snippet: Cadmium (Cd) is a heavy metal, which is widely used in the industry and daily life.. It has a long half-life, so large amounts of Cd can accumulate in humans and become toxic.. Chlorogenic acid (CGA) can eliminate free radicals and inhibit lipid peroxidation and is mainly used to prevent metal toxicity.

    Article Title: Structure characterization and in vitro immunomodulatory effects of a novel galactoglucomannan in cultivated Chinese cordyceps (Cordyceps sinensis).
    Article Snippet: Chinese cordyceps (Cordyceps sinensis) is a rare medicinal fungus with significant therapeutic value, and its cultivated variants have garnered increasing attention in recent years.. However, detailed structural and functional insights into cultivated Chinese cordyceps (CCC) polysaccharides remain limited.. In this study, a novel galactoglucomannan (CSWP-80) with a molecular weight (Mw) of 26.2 kDa was extracted and purified from CCC.

    Control:

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy.
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Protection Mechanisms Underlying Oral Administration of Chlorogenic Acid against Cadmium-Induced Hepatorenal Injury Related to Regulating Intestinal Flora Balance.
    Article Snippet: Cadmium (Cd) is a heavy metal, which is widely used in the industry and daily life.. It has a long half-life, so large amounts of Cd can accumulate in humans and become toxic.. Chlorogenic acid (CGA) can eliminate free radicals and inhibit lipid peroxidation and is mainly used to prevent metal toxicity.

    Article Title: Structure characterization and in vitro immunomodulatory effects of a novel galactoglucomannan in cultivated Chinese cordyceps (Cordyceps sinensis).
    Article Snippet: Chinese cordyceps (Cordyceps sinensis) is a rare medicinal fungus with significant therapeutic value, and its cultivated variants have garnered increasing attention in recent years.. However, detailed structural and functional insights into cultivated Chinese cordyceps (CCC) polysaccharides remain limited.. In this study, a novel galactoglucomannan (CSWP-80) with a molecular weight (Mw) of 26.2 kDa was extracted and purified from CCC.

    Comparison:

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy.
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Article Title: Protection Mechanisms Underlying Oral Administration of Chlorogenic Acid against Cadmium-Induced Hepatorenal Injury Related to Regulating Intestinal Flora Balance.
    Article Snippet: Cadmium (Cd) is a heavy metal, which is widely used in the industry and daily life.. It has a long half-life, so large amounts of Cd can accumulate in humans and become toxic.. Chlorogenic acid (CGA) can eliminate free radicals and inhibit lipid peroxidation and is mainly used to prevent metal toxicity.

    Article Title: Structure characterization and in vitro immunomodulatory effects of a novel galactoglucomannan in cultivated Chinese cordyceps (Cordyceps sinensis).
    Article Snippet: Chinese cordyceps (Cordyceps sinensis) is a rare medicinal fungus with significant therapeutic value, and its cultivated variants have garnered increasing attention in recent years.. However, detailed structural and functional insights into cultivated Chinese cordyceps (CCC) polysaccharides remain limited.. In this study, a novel galactoglucomannan (CSWP-80) with a molecular weight (Mw) of 26.2 kDa was extracted and purified from CCC.



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    Image Search Results


    AVA suppresses TLR4 expression in rat colon. photomicrographs depicting immunohistochemical staining of TLR4 in rat colon. A Control group, B LPS group, C LPS+AVA-treated group, D AVA alone treatment, and E quantification of TLR4 area percentage. The data (mean ± SD) underwent statistical evaluation utilizing one-way ANOVA with Tukey’s post hoc comparison. Statistical significance was indicated by “a” and “b,” representing differences from the normal control and LPS groups, respectively, at P < 0.05. LPS, lipopolysaccharide; AVA, avanafil; TLR4, Toll-like receptor 4

    Journal: Molecular Neurobiology

    Article Title: Therapeutic Repurposing of Avanafil Against Lipopolysaccharide-induced Depression and Autoimmune Hepatitis: Gut-brain-liver Axis Orchestration Via Regulation of TLR4/NF-κB/IDO and Nrf2/HO-1 Pathways

    doi: 10.1007/s12035-026-05854-4

    Figure Lengend Snippet: AVA suppresses TLR4 expression in rat colon. photomicrographs depicting immunohistochemical staining of TLR4 in rat colon. A Control group, B LPS group, C LPS+AVA-treated group, D AVA alone treatment, and E quantification of TLR4 area percentage. The data (mean ± SD) underwent statistical evaluation utilizing one-way ANOVA with Tukey’s post hoc comparison. Statistical significance was indicated by “a” and “b,” representing differences from the normal control and LPS groups, respectively, at P < 0.05. LPS, lipopolysaccharide; AVA, avanafil; TLR4, Toll-like receptor 4

    Article Snippet: The cells were then treated with anti-TLR4 antibody (bs-1021R - Bioss USA - 1:200), anti-ZO-1(1:1000 - Abcam - EPR19945-224), and anti-MMP-9 (GTX100458 - GeneTex Inc. - 1:200) overnight at 4 °C.

    Techniques: Expressing, Immunohistochemical staining, Staining, Control, Comparison

    AVA attenuates hepatic TLR4 expression. photomicrographs depicting immunohistochemical staining of TLR4 in rat liver. A Control group, B LPS group, C LPS+AVA-treated group, D AVA alone treatment, and E quantification of TLR4 area percentage. The data (mean ± SD) underwent statistical evaluation utilizing one-way ANOVA with Tukey’s post hoc comparison. Statistical significance was indicated by “a” and “b,” representing differences from the normal control and LPS groups, respectively, at P < 0.05. LPS, lipopolysaccharide; AVA, avanafil; TLR4, Toll-like receptor 4

    Journal: Molecular Neurobiology

    Article Title: Therapeutic Repurposing of Avanafil Against Lipopolysaccharide-induced Depression and Autoimmune Hepatitis: Gut-brain-liver Axis Orchestration Via Regulation of TLR4/NF-κB/IDO and Nrf2/HO-1 Pathways

    doi: 10.1007/s12035-026-05854-4

    Figure Lengend Snippet: AVA attenuates hepatic TLR4 expression. photomicrographs depicting immunohistochemical staining of TLR4 in rat liver. A Control group, B LPS group, C LPS+AVA-treated group, D AVA alone treatment, and E quantification of TLR4 area percentage. The data (mean ± SD) underwent statistical evaluation utilizing one-way ANOVA with Tukey’s post hoc comparison. Statistical significance was indicated by “a” and “b,” representing differences from the normal control and LPS groups, respectively, at P < 0.05. LPS, lipopolysaccharide; AVA, avanafil; TLR4, Toll-like receptor 4

    Article Snippet: The cells were then treated with anti-TLR4 antibody (bs-1021R - Bioss USA - 1:200), anti-ZO-1(1:1000 - Abcam - EPR19945-224), and anti-MMP-9 (GTX100458 - GeneTex Inc. - 1:200) overnight at 4 °C.

    Techniques: Expressing, Immunohistochemical staining, Staining, Control, Comparison

    Effect of FP and SFP on the TLR4/MyD88 signaling in blood vessels. (A) Representative images from immunohistochemical staining of TLR4 in blood vessels (×200). (B, C, E) Western blotting to determine the levels of TLR4, MyD88, NFκB p65 and its phosphorylation, IκBα and its phosphorylation, and IKKβ and its phosphorylation in blood vessels. (D) Changes in serum TNF-α and IL-6 levels. (F) Relative expression of TLR4, MyD88, NFκB p65, IκBα, and IKKβ mRNA. Compared with the WKY group, # p < 0.05, ## p < 0.01; compared with the SHR group, * p < 0.05, ** p < 0.01; compared with the FP group, ▲ p < 0.05, ▲▲ p < 0.01, n = 3.

    Journal: Ultrasonics Sonochemistry

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy

    doi: 10.1016/j.ultsonch.2026.107829

    Figure Lengend Snippet: Effect of FP and SFP on the TLR4/MyD88 signaling in blood vessels. (A) Representative images from immunohistochemical staining of TLR4 in blood vessels (×200). (B, C, E) Western blotting to determine the levels of TLR4, MyD88, NFκB p65 and its phosphorylation, IκBα and its phosphorylation, and IKKβ and its phosphorylation in blood vessels. (D) Changes in serum TNF-α and IL-6 levels. (F) Relative expression of TLR4, MyD88, NFκB p65, IκBα, and IKKβ mRNA. Compared with the WKY group, # p < 0.05, ## p < 0.01; compared with the SHR group, * p < 0.05, ** p < 0.01; compared with the FP group, ▲ p < 0.05, ▲▲ p < 0.01, n = 3.

    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Techniques: Immunohistochemical staining, Staining, Western Blot, Phospho-proteomics, Expressing